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Table 1 The yields of total cell protein, and total, soluble, insoluble GTFB, obtained from E. coli

From: Characterization of the 4,6-α-glucanotransferase GTFB enzyme of Lactobacillus reuteri 121 isolated from inclusion bodies

Induction temperature (°C) Total protein (mg/l culture) Total GTFB (mg/l culture) Soluble GTFB (mg/l culture) Insoluble GTFB (mg/l culture) % of insoluble GTFB in total IBs
18 168.5 ± 10.0 92.0 ± 5.5 6.0 ± 1.0 86.0 ± 5.0 82.5 ± 0.5
25 175.5 ± 17.5 95.0 ± 9.5 8.0 ± 1.5 87.0 ± 9.0 85.0 ± 0.5
30 224.5 ± 12.5 132.5 ± 7.5 1.5 ± 0.5 131.0 ± 7.0 83.0 ± 0.5
37 150.5 ± 9.0 86.0 ± 5.0 0.5 ± 0.0 85.5 ± 0.5 73.5 ± 0.5
  1. E.coli BL21 Star (DE3) cells grown at different temperatures, and the percentages of insoluble GTFB within the inclusion bodies (IBs) were determined by Bio-Rad protein assay and densitometric analysis of SDS-PAGE separated proteins. All cell samples were harvested at a culture OD600 of 1.8. The experiments were done in duplicate