Table 1 The yields of total cell protein, and total, soluble, insoluble GTFB, obtained from E. coli
Induction temperature (°C) | Total protein (mg/l culture) | Total GTFB (mg/l culture) | Soluble GTFB (mg/l culture) | Insoluble GTFB (mg/l culture) | % of insoluble GTFB in total IBs |
|---|---|---|---|---|---|
18 | 168.5 ± 10.0 | 92.0 ± 5.5 | 6.0 ± 1.0 | 86.0 ± 5.0 | 82.5 ± 0.5 |
25 | 175.5 ± 17.5 | 95.0 ± 9.5 | 8.0 ± 1.5 | 87.0 ± 9.0 | 85.0 ± 0.5 |
30 | 224.5 ± 12.5 | 132.5 ± 7.5 | 1.5 ± 0.5 | 131.0 ± 7.0 | 83.0 ± 0.5 |
37 | 150.5 ± 9.0 | 86.0 ± 5.0 | 0.5 ± 0.0 | 85.5 ± 0.5 | 73.5 ± 0.5 |