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Fig. 4 | BMC Biotechnology

Fig. 4

From: A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis

Fig. 4

Protocol for SLiCE from E. coli laboratory strains. a A “standard protocol” is available for various PCR fragments. b The “rapid protocol” is recommended for PCR fragments amplified as a single band. DpnI treatment efficiently reduces background colony formation. When a restriction enzyme-digested vector is used, DpnI should be inactivated for 15 min at 80 °C

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BMC Biotechnology

ISSN: 1472-6750

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