Figure 4

Immunoreactivity of antibodies in rat serum after immunization with the K8A, N11A, D55A mutant of the C-terminal domain of Phl p 1.0102 without the GST fusion tag (A-C) or PBS (D). Four rats were used for each immunization. Reactivity was tested against the non-GST fused K8A, N11A, D55A mutant (A and D), wild-type intact Phl p 1.0102 (B) and GST (C). Serum antibodies obtained from rats immunized with the IgE-hyporeactive mutant C-terminal domain of Phl p 1.0102 coupled to GST showed similar reactivity to the non-GST fused K8A, N11A, D55A mutant and the intact wild-type Phl p 1.0102, while the reactivity to GST was considerably higher (data not shown).