Figure 2From: Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytesThe target sequence and the constructed vectors. The genomic region around exon 3 of the IL11 gene was targeted by Cas9 nuclease (A), Cas9 nickase (B), or FokI-dCas9 (C) vectors. The target sequence of each gRNA is indicated by colored bases. The PAM sequence is underlined.Back to article page