Figure 1From: Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytesSchematic illustration of the all-in-one FokI-dCas9 vector construction system. Single gRNA-expressing vectors are constructed by the insertion of annealed oligonucleotides. Subsequently, the gRNA cassettes are assembled using Golden Gate cloning. Amp, ampicillin; Spec, spectinomycin; U6, human U6 promoter; CBh, chicken beta-actin short promoter.Back to article page