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Table 1 Result of Agrobacterium and Arabidopsis genome resequencing using the two different DNA fragmentation methods

From: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI

Species Fragmentation method Chromosome Number of reads Ratio of coverage (%) Average coverage depth (time)
Agrobacterium REPLI-g/MspJI Circular 1,380,029 99.9 64.3
   Linear 1,219,389 99.9 77.8
  Physical shearing Circular 546,292 99.8 27.4
   Linear 496,662 99.9 34.1
Arabidopsis REPLI-g/MspJI 1 4,611,463 97.3 36.4
   2 4,759,134 98 57.7
   3 4,702,657 98.3 47.9
   4 3,088,231 98 39.9
   5 4,222,266 98 37.8
  Physical shearing 1 5,116,095 98.9 41.5
   2 6,143,569 99.5 75
   3 6,083,236 99.5 62.4
   4 3,776,130 99.5 49.3
   5 4,980,370 99.4 45.1
  1. Ratio of coverage denotes the value for specific nucleotides located on each chromosome.