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Table 1 Result of Agrobacterium and Arabidopsis genome resequencing using the two different DNA fragmentation methods

From: A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI

Species

Fragmentation method

Chromosome

Number of reads

Ratio of coverage (%)

Average coverage depth (time)

Agrobacterium

REPLI-g/MspJI

Circular

1,380,029

99.9

64.3

  

Linear

1,219,389

99.9

77.8

 

Physical shearing

Circular

546,292

99.8

27.4

  

Linear

496,662

99.9

34.1

Arabidopsis

REPLI-g/MspJI

1

4,611,463

97.3

36.4

  

2

4,759,134

98

57.7

  

3

4,702,657

98.3

47.9

  

4

3,088,231

98

39.9

  

5

4,222,266

98

37.8

 

Physical shearing

1

5,116,095

98.9

41.5

  

2

6,143,569

99.5

75

  

3

6,083,236

99.5

62.4

  

4

3,776,130

99.5

49.3

  

5

4,980,370

99.4

45.1

  1. Ratio of coverage denotes the value for specific nucleotides located on each chromosome.