Figure 2From: Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila Constructed protein expression vectors of T. thermophila. A. Vector pVTtsfGFP includes the T. thermophila codon-adapted TtsfGFP expression cassette; B. Vector pVsfGFP carries a non-codon-adapted sfGFP expression cassette; C. Vector pEGFP includes the non-codon-adapted EGFP expression cassette. All vectors were derived from pVGF. MTT1 is a cadmium-inducible promotor. The transcription is terminated using an rpL29 termination sequence.Back to article page