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Figure 2 | BMC Biotechnology

Figure 2

From: Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila

Figure 2

Constructed protein expression vectors of T. thermophila. A. Vector pVTtsfGFP includes the T. thermophila codon-adapted TtsfGFP expression cassette; B. Vector pVsfGFP carries a non-codon-adapted sfGFP expression cassette; C. Vector pEGFP includes the non-codon-adapted EGFP expression cassette. All vectors were derived from pVGF. MTT1 is a cadmium-inducible promotor. The transcription is terminated using an rpL29 termination sequence.

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