Figure 5
PAGE and immunoblotting analysis of the purification process of recombinant HP-NAP expressed in E. coli . The whole cell lysate (W) and soluble fraction (S) of E. coli BL21(DE3) expressing HP-NAP, the pooled fraction (P) of the unbound fraction and wash fractions containing HP-NAP from DEAE Sephadex chromatography, the dialyzed HP-NAP solution (D), and the filtrated HP-NAP solution (F) were analyzed by SDS-PAGE (A), native-PAGE (B), and immunoblotting (C). Molecular weights (M) in kDa are indicated on the left of the stained gels and the blot. Data were representative of at least three independent experiments.