Figure 2
From: Expression of the Thermobifida fusca xylanase Xyn11A in Pichia pastoris and its characterization
Analysis of the accumulation of recombinant xylanases expressed in P. pastoris X-33 . Lane M: the molecular weight marker; A: SDS-PAGE of rXyn11A, Lane 1–8: supernatants of transformant harboring pPICZα-xynA obtained every 12 hours (12 h, 24 h, 36 h, 48 h, 60 h, 72 h, 84 h, 96 h), Lane 9: supernatant of transformant harboring pPICZα-A; B: SDS-PAGE of rXyn11A-(His)6, Lane 1–8: supernatants of transformant harboring pPICZα-xynA-(His)6 obtained every 12 hours (12 h, 24 h, 36 h, 48 h, 60 h, 72 h, 84 h, 96 h), Lane 9: supernatant of transformant harboring pPICZα-A. All samples containing 16 μL supernatant were loaded onto a 15% polyacrylamide gel.