Table 2 The observed C T differences and the calculated DNA concentrations (N) based on qPCR assays (Grain mix)
% waxy DNA | Grain mix | ||||||
|---|---|---|---|---|---|---|---|
C T wx a | C T Wx | ΔC T wx a -Wx | ΔC T STD | N wx a | N Wx | Calculated % waxy DNA (R 2  = 0.9918) | |
100 | 24.1 | 37.7 | −13.6 | - | 4.9 | 0.0 | 99.9 |
50 | 24.1 | 26.1 | −2.0 | 0.0 | 5.0 | 7.2 | 40.8 |
20 | 24.9 | 25.1 | −0.2 | 0.1 | 3.0 | 13.1 | 18.4 |
10 | 26.2 | 25.4 | 0.8 | 0.3 | 1.3 | 11.4 | 10.1 |
5 | 28.1 | 25.6 | 2.5 | 0.6 | 0.3 | 10.0 | 3.4 |
2 | 28.7 | 25.6 | 3.1 | 0.2 | 0.2 | 9.7 | 2.3 |
1 | 30.3 | 26.3 | 4.0 | 0.5 | 0.1 | 6.1 | 1.4 |
0.5 | 29.1 | 25.2 | 3.9 | 0.1 | 0.2 | 13.1 | 1.4 |
0 | - | 25.8 | −25.8 | 0.2 | 0.0 | 8.3 | 0.0 |