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Table 2 The observed C T differences and the calculated DNA concentrations (N) based on qPCR assays (Grain mix)

From: Development of a waxy gene real-time PCR assay for the quantification of sorghum waxy grain in mixed cereal products

% waxy DNA Grain mix
C T wx a C T Wx ΔC T wx a -Wx ΔC T STD N wx a N Wx Calculated % waxy DNA (R 2= 0.9918)
100 24.1 37.7 −13.6 - 4.9 0.0 99.9
50 24.1 26.1 −2.0 0.0 5.0 7.2 40.8
20 24.9 25.1 −0.2 0.1 3.0 13.1 18.4
10 26.2 25.4 0.8 0.3 1.3 11.4 10.1
5 28.1 25.6 2.5 0.6 0.3 10.0 3.4
2 28.7 25.6 3.1 0.2 0.2 9.7 2.3
1 30.3 26.3 4.0 0.5 0.1 6.1 1.4
0.5 29.1 25.2 3.9 0.1 0.2 13.1 1.4
0 - 25.8 −25.8 0.2 0.0 8.3 0.0
  1. The qPCR reactions were performed using 5 ng of DNA extracted from each grain mixture.
  2. The results are the means and standard deviations (STDs) of three independent assays.