% waxy DNA
|
Grain mix
|
---|
C
T
wx
a
|
C
T
Wx
|
ΔC
T
wx
a
-Wx
|
ΔC
T
STD
|
N
wx
a
|
N
Wx
|
Calculated % waxy DNA (R
2
= 0.9918)
|
---|
100
|
24.1
|
37.7
|
−13.6
|
-
|
4.9
|
0.0
|
99.9
|
50
|
24.1
|
26.1
|
−2.0
|
0.0
|
5.0
|
7.2
|
40.8
|
20
|
24.9
|
25.1
|
−0.2
|
0.1
|
3.0
|
13.1
|
18.4
|
10
|
26.2
|
25.4
|
0.8
|
0.3
|
1.3
|
11.4
|
10.1
|
5
|
28.1
|
25.6
|
2.5
|
0.6
|
0.3
|
10.0
|
3.4
|
2
|
28.7
|
25.6
|
3.1
|
0.2
|
0.2
|
9.7
|
2.3
|
1
|
30.3
|
26.3
|
4.0
|
0.5
|
0.1
|
6.1
|
1.4
|
0.5
|
29.1
|
25.2
|
3.9
|
0.1
|
0.2
|
13.1
|
1.4
|
0
|
-
|
25.8
|
−25.8
|
0.2
|
0.0
|
8.3
|
0.0
|
- The qPCR reactions were performed using 5 ng of DNA extracted from each grain mixture.
- The results are the means and standard deviations (STDs) of three independent assays.