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Table 2 The observed C T differences and the calculated DNA concentrations (N) based on qPCR assays (Grain mix)

From: Development of a waxy gene real-time PCR assay for the quantification of sorghum waxy grain in mixed cereal products

% waxy DNA

Grain mix

C T wx a

C T Wx

ΔC T wx a -Wx

ΔC T STD

N wx a

N Wx

Calculated % waxy DNA (R 2= 0.9918)

100

24.1

37.7

−13.6

-

4.9

0.0

99.9

50

24.1

26.1

−2.0

0.0

5.0

7.2

40.8

20

24.9

25.1

−0.2

0.1

3.0

13.1

18.4

10

26.2

25.4

0.8

0.3

1.3

11.4

10.1

5

28.1

25.6

2.5

0.6

0.3

10.0

3.4

2

28.7

25.6

3.1

0.2

0.2

9.7

2.3

1

30.3

26.3

4.0

0.5

0.1

6.1

1.4

0.5

29.1

25.2

3.9

0.1

0.2

13.1

1.4

0

-

25.8

−25.8

0.2

0.0

8.3

0.0

  1. The qPCR reactions were performed using 5 ng of DNA extracted from each grain mixture.
  2. The results are the means and standard deviations (STDs) of three independent assays.