Table 1 The observed C T differences and the calculated DNA concentrations (N) based on qPCR assays (DNA mix)
% waxy DNA | DNA mix | ||||||
|---|---|---|---|---|---|---|---|
|  | C T wx a | C T Wx | ΔC T wx a -Wx | ΔC T STD | N wx a | N Wx | Calculated % waxy DNA (R 2  = 0.9991) |
100 | 24.3 | 36.0 | −11.7 | - | 4.5 | 0.0 | 99.8 |
50 | 25.2 | 27.7 | −2.5 | 0.0 | 2.4 | 2.4 | 49.7 |
20 | 26.7 | 26.9 | −0.2 | 0.4 | 0.9 | 4.1 | 18.2 |
10 | 27.4 | 26.6 | 0.8 | 0.2 | 0.6 | 4.9 | 10.1 |
5 | 28.6 | 27.2 | 1.4 | 1.1 | 0.2 | 3.4 | 6.8 |
2 | 29.5 | 26.9 | 2.6 | 0.5 | 0.1 | 4.3 | 3.2 |
1 | 30.6 | 26.6 | 4.0 | 0.7 | 0.1 | 5.1 | 1.3 |
0.5 | 32.0 | 26.4 | 5.6 | 0.5 | 0.03 | 5.7 | 0.5 |
0 | - | 26.1 | −26.1 | 0.2 | 0.0 | 7.1 | 0.0 |