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Table 1 The observed C T differences and the calculated DNA concentrations (N) based on qPCR assays (DNA mix)

From: Development of a waxy gene real-time PCR assay for the quantification of sorghum waxy grain in mixed cereal products

% waxy DNA DNA mix
  C T wx a C T Wx ΔC T wx a -Wx ΔC T STD N wx a N Wx Calculated % waxy DNA (R 2= 0.9991)
100 24.3 36.0 −11.7 - 4.5 0.0 99.8
50 25.2 27.7 −2.5 0.0 2.4 2.4 49.7
20 26.7 26.9 −0.2 0.4 0.9 4.1 18.2
10 27.4 26.6 0.8 0.2 0.6 4.9 10.1
5 28.6 27.2 1.4 1.1 0.2 3.4 6.8
2 29.5 26.9 2.6 0.5 0.1 4.3 3.2
1 30.6 26.6 4.0 0.7 0.1 5.1 1.3
0.5 32.0 26.4 5.6 0.5 0.03 5.7 0.5
0 - 26.1 −26.1 0.2 0.0 7.1 0.0
  1. The qPCR reactions were performed using 5 ng of DNA mix or DNA extracted from grain mix.
  2. Mean and standard deviation (STD) values from three independent assays.