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Table 1 The observed C T differences and the calculated DNA concentrations (N) based on qPCR assays (DNA mix)

From: Development of a waxy gene real-time PCR assay for the quantification of sorghum waxy grain in mixed cereal products

% waxy DNA

DNA mix

 

C T wx a

C T Wx

ΔC T wx a -Wx

ΔC T STD

N wx a

N Wx

Calculated % waxy DNA (R 2= 0.9991)

100

24.3

36.0

−11.7

-

4.5

0.0

99.8

50

25.2

27.7

−2.5

0.0

2.4

2.4

49.7

20

26.7

26.9

−0.2

0.4

0.9

4.1

18.2

10

27.4

26.6

0.8

0.2

0.6

4.9

10.1

5

28.6

27.2

1.4

1.1

0.2

3.4

6.8

2

29.5

26.9

2.6

0.5

0.1

4.3

3.2

1

30.6

26.6

4.0

0.7

0.1

5.1

1.3

0.5

32.0

26.4

5.6

0.5

0.03

5.7

0.5

0

-

26.1

−26.1

0.2

0.0

7.1

0.0

  1. The qPCR reactions were performed using 5 ng of DNA mix or DNA extracted from grain mix.
  2. Mean and standard deviation (STD) values from three independent assays.