DNA modifications in somatic embryos. (A) Long-distance PCR for the Cas9 gene in recovered events with 01g + 11gDDM1 and 07g14530. Marker is a 1 Kb DNA ladder. Asterisks (*) indicate events with an intact Cas9. (B) Modifications were detected in three events transformed with the 01g + 11gDDM1 vector. At the initial time-point, modifications were only detected in event 24. When samples were taken approximately 2 weeks later, modifications were detected in all three events. (C) Modifications were detected in 14 out of 16 individual regenerating embryos from event 24.