Table 1 Purification of cellulase from Bacillus vallismortis RG-07
Purification steps | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification fold |
|---|---|---|---|---|---|
Crude | 3,81,788 | 120.75 | 3161.81 | 100 | 1.0 |
Ammonium sulphate | 3,20,190.9 | 21.54 | 14864.94 | 83.8 | 4.7 |
Q-Sepharose | 2,88089.4 | 7.79 | 36981.95 | 75.4 | 11.6 |
Sephadex G-75 | 109,968.9 | 0.89 | 123560.56 | 28.8 | 39.1 |