Figure 3
The auto-induction of Origami 2 (pET28-HisTag-tPA-RGDS) at various temperatures. A, Recombinant tPA after auto-induction at 25°C. Lane 1, protein marker; lane 2, lysate; lane 3, supernatant; lane 4, flow-through; lanes 5 and 6, washes with 20 and 40 mM imidazole, respectively; lane 7, elution with 200 mM imidazole. B, Recombinant tPA after auto-induction at 33°C. Lane 1, protein marker; lane 2, supernatant; lane 3, flow-through; lanes 4 and 5, washes with 20 and 40 mM imidazole, respectively; lane 6, elution with 200 mM imidazole. C, Recombinant tPA after auto-induction at 35°C. Lane 1, protein marker; lane 2, lysate; lane 3, supernatant; lane 4, flow-through; lanes 5 and 6, washes with 20 and 40 mM imidazole, respectively; lane 7, elution with 200 mM imidazole. D, Recombinant tPA after auto-induction at 37°C. Lane 1, lysate; lane 2, supernatant; lane 3, flow-through; lanes 4 and 5, washes with 20 and 40 mM imidazole, respectively; lane 6, elution with 200 mM imidazole;Lane 7, protein marker.