Figure 5

Molecular mass determination of mAP by size exclusion chromatography. mAP and six molecular mass standard proteins (peak a, ferritin; peak b, adolase; peak c, albumin; peak d, ovalbumin; peak e, chymotrypsinogen A; and peak f, RNase A) were each subjected to size exclusion chromatography on a Superose 6 HR column that had been pre-equilibrated with 25 mM Tris–HCl buffer containing 150 mM NaCl at pH 7.6. The molecular mass of mAP was estimated by comparing the retention time of mAP with the standard curve (plot of retention time versus logMW) obtained for the molecular mass standard proteins (inset).