Multiple alignment of the deduced amino acid sequence of the mAP gene derived from the metagenome with other alkaline phosphatases (APs). APs are identified by their GenBank or PDB accession numbers: Sphingomonas sp. strain BSAR-1 AP (SPAP, ABL96598), Escherichia coli AP (ECAP, BAE76164), Vibrio sp. G15-21 AP (VAP, AAK94204), Antarctic bacterium TAB5 AP (AAP, CAB82508), shrimp AP (SAP, PDB 1SHQ), human placenta AP (HPAP, PDB 1ZEB), Shewanella sp. AP1 AP (SCAP, BAB85685), and Sinorhizobium meliloti 1021 AP (SMAP, NP 385195). Highly conserved amino acid residues are highlighted in black, and gaps are denoted by dots. Gray-shaded amino acids are conserved in at least six of the nine APs shown. The amino acid residues forming the metal binding site and catalytic residues of mAP are denoted by asterisks (★) and closed circle (●), respectively. Numbers along the sequences indicate the positions of the amino acid residues starting from the initial Met for each AP.