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Figure 7 | BMC Biotechnology

Figure 7

From: Modifications of cysteine residues in the transmembrane and cytoplasmic domains of a recombinant hemagglutinin protein prevent cross-linked multimer formation and potency loss

Figure 7

RP-HPLC profiles for the H3 rHA proteins. Each H3 rHA was analyzed in duplicate. Representative chromatograms from the day 0 and 28 time points are provided. rHAs were incubated with 25 mM DTT for at least 30 minutes prior to analysis. ~25 μg of each rHA was injected onto a Poros R1 column and an acetonitrile gradient applied.

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