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Table 1 High-affinity ligands of microvirin from the CFG's glycoarray analysis

From: Detection of distinct glycosylation patterns on human γ-glutamyl transpeptidase 1 using antibody-lectin sandwich array (ALSA) technology

Glycan # Composition Fluorescence (%) a
212 Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 100.0
215 Manα1-2Manα1-2Manα1-6(Manα1-3)Manα-Sp9 78.1
216 Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 72.9
208 Manα1-2Manα1-2Manα1-3Manα-Sp9 63.8
210 Manα1-2Manα1-3Manα-Sp9 57.7
316 Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manα-Sp9 48.8
209 Manα1-2Manα1-6(Manα1-2Manα1-3)Manα-Sp9 39.5
317 Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manα-Sp9 34.9
211 Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 31.7
213 Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 19.8
  1. aExpressed as a percentage of the highest-affinity ligand, which exhibited a fluorescence reading of 19,838 RFU at 200 μg/mL microvirin.