Figure 1

Glycan array analysis of MVN CRD specificity. (A) Binding profile of MVN at 200 μg/mL, using the CFG array V5.0 harboring 611 different carbohydrate structures. Relative fluorescence units reflect relative affinities toward the corresponding glycan. Glycans bound by MVN are indicated by their CFG array numbers (see Table 1). The diagram is based on the primary CFG data spreadsheets, which can be accessed at http://www.functionalglycomics.org/glycomics/publicdata/selectedScreens.jsp (Glycan Array #2340). (B) MVN glycan array binding at 2, 20, 200 μg/mL as ranked by fluorescence intensity (from 200 μg/mL data set) and apparent binding affinity as determined by Outlier Motif Analysis. Binding was considered positive if the RFU value was greater than the primary fluorescence threshold, calculated to be 2,319 RFU (see Figure 2). All 11 carbohydrates with above-threshold RFU values contained a Manα(1-2)Man- determinant, and the four shown illustrate the binding affinity differences that could be attributed to presence of the central arm Manα(1-2)Man-disaccharide. Symbols: blue box, GlcNAc; green circle, mannose.