Table 2 Purification of cellobiose dehydrogenase from Sclerotium rolfsii
Purification step | CDH activity (Units) | Total protein (mg) | Specific activity (U/mg) | Purification fold | Yield (%) |
|---|---|---|---|---|---|
Crude extract | 1173 | 1112 | 1.1 | 1.0 | 100 |
IEX (Q Sepharose XL) | 974 | 456 | 2.1 | 2.0 | 83 |
(NH4)2SO4 precipitation | 750 | 398 | 1.9 | 1.8 | 64 |
HIC (Phenyl HP) | 661 | 56 | 11.9 | 11.3 | 56 |