Table 1 dynamic binding capacity determined at 5% breakthrough and specific enzyme activity in the elute
Column | pH value | Dynamic binding capacity (Units) | Specific activity (U/mg) |
|---|---|---|---|
Q Sepharose XL | 4.5 | 140 | 1.6 |
Q Sepharose XL | 5.0 | 180 | 1.5 |
Q Sepharose XL | 5.5 | 210 | 1.5 |
Q Sepharose XL | 6.0 | > 350 | 1.5 |
DEAE Sepharose FF | 5.0 | 62 | 1.0 |