A novel serine hydroxymethyltransferase from Arthrobacter nicotianae: characterization and improving catalytic efficiency by rational design

Table 3 Primers used for plasmid construction and the site-directed mutagenesis

Primers	5′ to 3′	Purpose or references
DP-F	RTGAMCMCKNYATC	This study
DP-R	YTACCCSWGSTCSK	This study
An--F	CGGGATCCATGAGCAACCAGACTTTTGAATC	This study
An--R	CGGAATTCCTACTCGGAAACCTTTGGCAGGT	This study
I249L-F	CCGCGTGGCGGCCTGATTCTGTCGA	This study
I249L-R	CAGGCCGCCACGCGGACCAGCCAAC	This study

IUPAC ambiguity codes: M = A/C, R = A/G, W = A/T, S = G/C, Y = C/T, K = G/T and N = A/G/C/T. I indicates inosine. Restriction sites BamH1 and EcoRI in primers An--F/R and the mutated site are underlined; Start and stop codons are marked in bold; site-directed mutagenesis site is underline in I249L-F and I249L-R.

ISSN: 1472-6750