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Table 1 Steady-state kinetic parameters for the wild-type An SHMT and the mutant

From: A novel serine hydroxymethyltransferase from Arthrobacter nicotianae: characterization and improving catalytic efficiency by rational design

Enzyme

Km (mM)

k cat (min -1)

k cat / Km (min -1M -1)

WT

57.86 ± 0.07

96.02 ± 0.12

1.66 × 103

I249L

61.95 ± 0.13

285.3 ± 0.15

4.61 × 103

  1. Note. The data are the average of three replicates.
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BMC Biotechnology

ISSN: 1472-6750

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