Figure 5From: Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coliSDS-PAGE analysis of recombinant 6-His-hCNTF purification. M: protein marker; 1: crude cell lysate; 2: soluble fraction; 3: Ni-IDA purified hCNTF; 4: size exclusion chromatography purified hCNTF. The gel was stained with Coomassie Blue dye.Back to article page