Figure 3
From: Improvement of an antibody-enzyme coupling yield by enzyme surface supercharging
Use of a hEPl scC112S-antibody conjugate for the detection of EGF. Epidermal growth factor (in E. coli cell lysate as matrix) was detected using a sandwich ELISA and an in-house produced donkey-anti-rabbit/hEPl scC112S conjugate as secondary antibody. Signal was generated by addition of 50 μmol/L GD4K-na and an end-point measurement was performed after 4 hours of incubation at 37°C. The limit of detection (S/B >3xSD) was 15.63 pg EGF. Errors = SD, n = 3.