Real time quantitative PCR results of p21 (A), FST (B), CDK2 (C), MSTN (D), Akt I (E), ActRIIB (F)and p57 (G) mRNA expression during proliferation of ovine primary myoblast. Cells cultured in 6-well plates for eight days and harvested at days 2, 4, 6 and 8. The mRNA concentrations were quantified by real-time quantitative PCR. The amount of mRNAs was normalized to the amount GAPDH mRNA. Data were analyzed by one-way ANOVA (P < 0.05, n = 8). Statistical differences are indicated: a > b > c > d.