Box shade diagram showing similarity of deduced amino acid sequences encoding ovine follistatin in primary myoblast cells. A, Multiple sequence alignment of proteins was carried out using the DNAMAN 6.0 method based on amino acid sequences of follistatins from Ovisaries (NP_001244022.1), Bos Taurus (NP_786995.2), Capra hircus (ADJ53355.1) and Homo sapiens (BC004107.2). B, Gene map of recombinant plasmid pAAV-CFS-FLAG: ITR: inverted terminal repeat; CMV promoter: Cytomegalovirus promoter; FST: coding region for follistatin; IRES: internal ribosome entry site; GFP: green fluorescent protein; hGHpA: human growth hormone (hGH) polyA signal; C, Detection of FST-FLAG proteins in transduced and normal ovine primary myoblasts by western blot analysis. 15 mg of total protein was resolved by 12% SDS-PAGE. Monoclonal anti-Flag (Sigma) were the primary antibody used in the western blot.