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Table 4 Kinetic parameters for binding of scFv K1, HM-1 and HYI to nmAb-KT measured by SPR analysis

From: An improved phage-display panning method to produce an HM-1 killer toxin anti-idiotypic antibody

Ligand

Analytes

kon (× 104 M-1 s-1)

koff (× 10-4 s-1)

Ka (× 107 M-1)

Kd (× 10-8 M)

nmAb-KT

scFv K1

1.02

4.71

2.17

4.62

nmAb-KT

HM-1

4.92

3.31

14.80

0.674

nmAb-KT

HYI

ND

ND

ND

ND

  1. Ligand nmAb-KT was immobilized on the surface of a CM5 sensor chip, and scFv K1, HM-1 peptide or HYI peptide, were then injected over this immobilized surface in the presence of HBS-EP buffer. The affinity constant (Ka) and dissociation constant (Kd) were obtained from the ratio of the association (kon) and dissociation (koff) rate constants derived from global fits of response against time data to a 1:1 Langmuir binding model. Ka = kon/koff; Kd = koff/kon; ND, signals not detected. HM-1 analyte was used for the positive control and HYI analyte was used for the negative control experiment [26].
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BMC Biotechnology

ISSN: 1472-6750

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