Figure 7
From: An improved phage-display panning method to produce an HM-1 killer toxin anti-idiotypic antibody
Functional characterization of recombinant scFvs by measuring in vitro antifungal activity. A. Biological activity of purified five clones scFv (K1 - K5). The effect of recombinant scFvs on the growth of S. cerevisiae A451 cells was measured by a cell suspension assay as described in Methods. Gray bars: Yeast Growth (%); black bars: Protein at 280 nm. B. Elution profile for scFv purification by using an anti-E-tag Sepharose column and the biological activity of the selected clone (scFv K1) against the growth of S. cerevisiae A451. Purified scFv K1 proteins per fraction (fr) were detected and visualized by SDS-PAGE and Western blotting with HRP-antiE-tag conjugate. A clear band of the expected size (30 kDa) for E-tag containing scFv was present in the purified elution fractions. C. Biological activity of scFv K1 against C. albicans ATCC 10231. For all cases fungal growth was measured at 600 nm. The results were means of duplicates.