Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard) × Brassica napus (oilseed rape) hybrid populations

Table 2 Transgenic mitigation study:percent germination and transgenic segregation frequencies under non-competitive and competitive conditions.

	Parent Populations				Progeny Populations: Competition				Progeny Populations: No Competition
Line	Seeds Plated	Germination (%)	Transgenic Ratio (%)	MS	Seeds Sown	Germination (%)	Transgenic Ratio (%)	MS	Seeds Sown	Germination (%)	Transgenic Ratio (%)	MS
BN	125	100	0	K	125	93.9	1.4	IJ	125	89.1	2.8	HI
BR	125	100	0	K	125	92.2	7.3	G	125	89.8	5.7	GH
GT1	125	80.7	55.1	C	125	89.9	38.5	D	125	88.6	36.8	D
GT5	125	79.3	63.9	BC	125	88.9	34	D	125	93.3	37.1	D
GT9	125	90.7	32.1	DE	125	90.7	10.3	FG	125	89.8	10.4	FG
TM(H)	125	86.4	100	A	125	81.6	77.7	ABC	125	82.6	88.3	AB
TM(B)	125	87.2	22.4	EF	125	84.3	0.4	JK	125	87.3	0.7	IJK

Percent germination and transgenic frequencies were calculated for the parental (BN: B. napus; BR: B. rapa; TM(H) and hybrid BC₁/F₂ populations (GT1, GT5, GT9, and TM) pre-season and post-harvest. Transgene persistence data reported here only applies to gene flow from GT hybrid plots and not TM hybrid or homozygous plots. Column MS represents transgenic ratio mean separation obtained by Fisher's LSD (P < 0.0001). Data was rank transformed and SAS software was used for data analysis.

ISSN: 1472-6750