Figure 9

Tamarind seed endosperm specificity. Specificity of staining as illustrated by the inability of XGBM XG-34/1-X to stain tamarind seed integument (arrows) that is rich in fucosylated xyloglucan. Images show a section seen under visible light (A), the fluorescence of the same section (B) and a merged image thereof (C). While the module stained endosperm cells, the integument cells covering endosperm remained unstained. As a control, integument cells stained well with the monoclonal antibody CCRC-M1 (D) and the CBM FXG-14b (E) that are both specific for fucosylated xyloglucan. XGBM XG-34, XG-34/2-I and XG-34/2-VI all showed a staining pattern similar to that of XG-34/1-X (data not shown). Bar lines = 200 μm