Protein sequences. Sequence alignment of XG-34, the three evolved modules XG-34/1-X, XG-34/2-I and XG-34/2-VI and CBM4-2. Residue numbering is in agreement with Simpson et al . Dots indicate identity with XG-34 sequence. The close proximity of residue 112 (green), mutated in evolved variants of XG-34, to residues 110 (blue) and 69 (red) that are important for carbohydrate binding in proteins based on the scaffold [15, 22], is highlighted on a structure model of XG-34/1-X.