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Figure 1 | BMC Biotechnology

Figure 1

From: Characterization of unknown genetic modifications using high throughput sequencing and computational subtraction

Figure 1

Construct-derived sequences found in the transgenic EST libraries generated using the ABF3 rice line and SunUp papaya. 15 sequences were found in the rice library, whereas the SunUp papaya cDNA collection contained 23 construct-derived sequences. Two versions of the papaya ringspot virus coat protein (PRSVcp) transcripts were found, and labeled in green are sequences from the cucumber mosaic virus coat protein (CMVcp) gene. When present in the sequences, poly(A) tails have been indicated and the sequences have been labeled with their GenBank EST accession numbers. Construct maps were modified from [18, 19, 21] and http://www.agbios.com/. Ubi1 - maize ubiquitin promoter 1. ABF3 - abscisic acid responsive elements-binding factor 3. 3'pinII - 3' region of potato proteinase inhibitor II. 35S - Cauliflower Mosaic Virus (CaMV) P35S promoter. bar - phosphinothricin acetyltransferase. 3'nos - 3' region of nopaline synthase. 5'nos - 5' region of nopaline synthase. nptII - neomycin phosphotransferase II. PRSVcp - Papaya Ringspot Virus coat protein. t35S - CaMV P35S terminator. UidA - beta glucuronidase.

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