Vector
|
Frequency of Read-througha
|
---|
pHIV-1SDmSE
|
9.05 ± 0.93 × 10-3
|
pRK2
|
8.70 ± 2.41 × 10-3
|
pRK2prt
|
8.57 ± 3.50 × 10-2
|
pRK2DSE
|
1.35 ± 0.41 × 10-2
|
pRK2DSEprt
|
8.72 ± 1.42 × 10-2b
|
- aReal time PCR was used to determine the frequency of polyadenylation signal read-through in 293T cells transduced with the vector, pcDNA3.1Tat101ml and pHCMVrevnlwhvpre. Results are Mean ± SD.
- bP < 0.05 vs pHIV-1SDmSE, pRK2 and pRK2DSE, t-test