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Table 1 Rate of polyadenylation read-through (n = 3)

From: Refinement of lentiviral vector for improved RNA processing and reduced rates of self inactivation repair

Vector

Frequency of Read-througha

pHIV-1SDmSE

9.05 ± 0.93 × 10-3

pRK2

8.70 ± 2.41 × 10-3

pRK2prt

8.57 ± 3.50 × 10-2

pRK2DSE

1.35 ± 0.41 × 10-2

pRK2DSEprt

8.72 ± 1.42 × 10-2b

  1. aReal time PCR was used to determine the frequency of polyadenylation signal read-through in 293T cells transduced with the vector, pcDNA3.1Tat101ml and pHCMVrevnlwhvpre. Results are Mean ± SD.
  2. bP < 0.05 vs pHIV-1SDmSE, pRK2 and pRK2DSE, t-test
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BMC Biotechnology

ISSN: 1472-6750

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