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Table 1 Rate of polyadenylation read-through (n = 3)

From: Refinement of lentiviral vector for improved RNA processing and reduced rates of self inactivation repair

Vector Frequency of Read-througha
pHIV-1SDmSE 9.05 ± 0.93 × 10-3
pRK2 8.70 ± 2.41 × 10-3
pRK2prt 8.57 ± 3.50 × 10-2
pRK2DSE 1.35 ± 0.41 × 10-2
pRK2DSEprt 8.72 ± 1.42 × 10-2b
  1. aReal time PCR was used to determine the frequency of polyadenylation signal read-through in 293T cells transduced with the vector, pcDNA3.1Tat101ml and pHCMVrevnlwhvpre. Results are Mean ± SD.
  2. bP < 0.05 vs pHIV-1SDmSE, pRK2 and pRK2DSE, t-test
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BMC Biotechnology

ISSN: 1472-6750

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