|
Pseudotype
|
Hepatocytes/mm2
|
NPC/mm2
|
Specificity
|
|---|
|
wtGP64 (n = 5)
|
9.9 ± 2.7
|
79.3 ± 12.1
|
0.125
|
|
Sendai-GP64/wtGP64 (n = 7)
|
9.5 ± 4.7
|
24.6 ± 19.6 a
|
0.386
|
|
Sendai-spGP64/wtGP64 (n = 4)
|
5.6 ± 1.4b
|
47.5 ± 19.4b
|
0.118
|
- Lentiviral vectors pseudotyped with wild type GP64 or mixes of wild type GP64 and Sendai-GP64 fusion proteins were generated and concentrated as described.
- Mice were injected in the portal vein with 0.5 × 108 HepG2 transducing units of each virus. After one week frozen fixed liver sections were prepared from the medial and left lobes and GFP positive cells were counted using a fluorescence microscope.
- NPC: nonparenchymal liver cells.
- Specificity was calculated as the ratio of GFP positive hepatocytes/mm2 to GFP positive NPC/mm2 and indicates relative affinity for hepatocytes.
- a represents a significant difference (p < 0.005) between wtGP64 and Sendai-GP64/wtGP64 transduced animals. b represents a significant difference (p < 0.05) between wtGP64 and Sendai-spGP64/wtGP64 transduced animals.
