Lentiviral vectors pseudotyped with wild type GP64 or mixes of wild type GP64 and Sendai-GP64 fusion proteins were generated and concentrated as described.
Mice were injected in the portal vein with 0.5 × 108 HepG2 transducing units of each virus. After one week frozen fixed liver sections were prepared from the medial and left lobes and GFP positive cells were counted using a fluorescence microscope.
NPC: nonparenchymal liver cells.
Specificity was calculated as the ratio of GFP positive hepatocytes/mm2 to GFP positive NPC/mm2 and indicates relative affinity for hepatocytes.
a represents a significant difference (p < 0.005) between wtGP64 and Sendai-GP64/wtGP64 transduced animals. b represents a significant difference (p < 0.05) between wtGP64 and Sendai-spGP64/wtGP64 transduced animals.