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Table 2 Specificity of virus produced with different ratios of Sendai-GP64 to wild type GP64.

From: Reduction of liver macrophage transduction by pseudotyping lentiviral vectors with a fusion envelope from Autographa californicaGP64 and Sendai virus F2 domain

Ratio

HeLa

HepG2

Specificity

2:1

2.2 × 105 ± 1.7 × 105

1.6 × 106 ± 1.2 × 106

7.4

9:1

4.5 × 104 ± 2.2 × 104

7.1 × 105 ± 2.1 × 105

15.8

29:1

1.8 × 104 ± 1.1 × 104

5.1 × 105 ± 2.7 × 105

28.9

99:1

2.5.103 ± 1.5 × 103

7.7 × 104 ± 1.6 × 104

30.7

  1. The ratio's 2:1, 9:1, 29:1, 99:1 refer to the relative amount of Sendai-GP64 to GP64 plasmid used for transfection during virus production. Viral titers were determined using unconcentrated lentiviral vectors from at least three different virus preparations.
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BMC Biotechnology

ISSN: 1472-6750

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