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Table 1 Growth kinetics of NT2 cell expansion as 3D-aggregates using different culture strategies.

From: Integrating human stem cell expansion and neuronal differentiation in bioreactors

Strategy μ (day-1) FI Xmax (×105 cell/mL)
SP-0.4B n. a. n. a. 0.63 ± 0.11 *
SP-1B 0.51 ± 0.01 7.14 ± 0.86 * 6.64 ± 1.57
SP-4B 0.39 ± 0.02 2.12 ± 0.03 8.48 ± 0.11
SP-4FB 0.52 ± 0.06 4.30 ± 0.33 * 17.19 ± 1.30 *
SP-4ME 0.41 ± 0.06 4.56 ± 0.04 * 18.25 ± 0.18 *
BR-4ME 0.37 ± 0.03 4.10 ± 0.41 16.25 ± 0.16
  1. Apparent growth rate (μ), fold increase (FI) and maximum cell concentration values (Xmax) of NT2 cells cultured in spinner vessel (SP) or in bioreactor (BR); with inoculum densities of 0.4 × 105 (SP-0.4) 1 × 105 (SP-1) or 4 × 105 cell/mL (SP-4, BR-4); in batch (B), fed-batch (FB) and media-exchange (ME) culture operation mode. Results are expressed as mean ± SEM from n = 2 independent experiments. n. a. - not applicable. *Indicates significant statistical difference (p-value < 0.05) from the SP-4B mean values of μ, FI and Xmax by the one-way ANOVA analysis with a Scheffé post-hoc multiple comparison test.