Integrating human stem cell expansion and neuronal differentiation in bioreactors

BMC Biotechnology

Table 1 Growth kinetics of NT2 cell expansion as 3D-aggregates using different culture strategies.

Strategy	μ (day^-1)	FI	X_max (×10⁵ cell/mL)
SP-0.4B	n. a.	n. a.	0.63 ± 0.11 *
SP-1B	0.51 ± 0.01	7.14 ± 0.86 *	6.64 ± 1.57
SP-4B	0.39 ± 0.02	2.12 ± 0.03	8.48 ± 0.11
SP-4FB	0.52 ± 0.06	4.30 ± 0.33 *	17.19 ± 1.30 *
SP-4ME	0.41 ± 0.06	4.56 ± 0.04 *	18.25 ± 0.18 *
BR-4ME	0.37 ± 0.03	4.10 ± 0.41	16.25 ± 0.16

Apparent growth rate (μ), fold increase (FI) and maximum cell concentration values (X_max) of NT2 cells cultured in spinner vessel (SP) or in bioreactor (BR); with inoculum densities of 0.4 × 10⁵ (SP-0.4) 1 × 10⁵ (SP-1) or 4 × 10⁵ cell/mL (SP-4, BR-4); in batch (B), fed-batch (FB) and media-exchange (ME) culture operation mode. Results are expressed as mean ± SEM from n = 2 independent experiments. n. a. - not applicable. *Indicates significant statistical difference (p-value < 0.05) from the SP-4B mean values of μ, FI and Xmax by the one-way ANOVA analysis with a Scheffé post-hoc multiple comparison test.

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