Quantification of the hCD4 marker and the eGFP expression before MACS. A population of T2EC not transfected (A) or transfected with either the pT2MIK empty plasmid (B) or the pT2MIK-eGFP expression plasmid (C) were labeled with an APC-conjugated antibody that recognizes the hCD4. Labeled cell fluorescence was analyzed by flow cytometry (FACS) one day after transfection. The positive fluorescence threshold is fixed in order to have 99% of the negative cells (i.e. untransfected and unlabeled cells) below this threshold. A 1% value is, hence, considered as null.