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Figure 4 | BMC Biotechnology

Figure 4

From: A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors

Figure 4

Quantification of the hCD4 marker and the eGFP expression before MACS. A population of T2EC not transfected (A) or transfected with either the pT2MIK empty plasmid (B) or the pT2MIK-eGFP expression plasmid (C) were labeled with an APC-conjugated antibody that recognizes the hCD4. Labeled cell fluorescence was analyzed by flow cytometry (FACS) one day after transfection. The positive fluorescence threshold is fixed in order to have 99% of the negative cells (i.e. untransfected and unlabeled cells) below this threshold. A 1% value is, hence, considered as null.

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BMC Biotechnology

ISSN: 1472-6750

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