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Table 1 Summary of success frequencies and purities of the in situ purification of 90 recombinant S. pneumoniae proteins.

From: High quality protein microarray using in situprotein purification

Protein class Number of proteins Purification (%) >90% relative purity (%) <90% relative purity (%)
Membrane 33 24 (72.7%) 19 (57.6%) 5 (15.2%)
Non-membrane 57 49 (86.0%) 47 (82.5%) 2 (3.5%)
Total 90 73 (81.1%) 66 (73.3%) 7 (7.8%)
  1. Immobilized His-tagged proteins and E. coli endogenous proteins were detected using an Alexa Fluor 555 fluorophore conjugated anti-His-tag antibody, and rabbit anti-E. coli antibody followed by incubation with ATTO 550 conjugated goat anti-rabbit antibody. The relative purity was calculated using the Equation 1 (text).