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Figure 5 | BMC Biotechnology

Figure 5

From: Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins

Figure 5

Production of Neu5Ac from GlcNAc using immobilized GST-GlcNAc 2-epimerase-5D and GST-Neu5Ac aldolase-5R. Conversion (upper panel) and time-course (lower panel) of GlcNAc, ManNAc, pyruvate, and Neu5Ac (sialic acid) concentrations in the synthesis of Neu5Ac from GlcNAc and pyruvate. Pyruvate was added at (a) 8 h (0.6 ml, 5 M), (b) 16 h (1.2 ml, 5 M), (c) 32 h (1.2 ml, 5 M), and (d) 50 h (0.6 ml, 5 M), and the temperature shifted from 30°C to 20°C at 32 h. Other reaction conditions are indicated in Methods.

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BMC Biotechnology

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