Figure 5

Inhibition ELISA of anti-Aflatoxin B1 antibody. Various 1:2 dilutions of soluble Aflatoxin B1 from 5.0–0.039 μg/ml in 2% MPBS were incubated with three selected Phage (C3, C5, D2) at 37°C for 30 min before adding into wells of Immuno 96 MicroWell™ Plates, coated with 4 μg/ml BSA-conjugated AlatoxinB1. After 1 hr of incubation, the plates were washed three times with PBS-T followed by 3 times with PBS. Bound phage were detected with anti-M13 phage-horseradish peroxidase (HRP) conjugate, using ABTS (2,2-azino-di-3-ethyl-benzthiazoine-6-sulfonate) as a substrate. The average absorbance at a wavelength of 405 nm and S.D. are shown.