Figure 6
From: Interfering ribonucleic acids that suppress expression of multiple unrelated genes
(a) VEGF and ICAM expression in ARPE-19 cells after transfection with inosine containing CODEMIRs (#100–102). ARPE-19 cells were transfected with 40 nM duplex RNA and VEGF-A (ELISA) or ICAM-1 (FACS) were assayed 48 hours post-transfection. (b) Comparison of VEGF-A suppressive activity of CODEMIRs containing inosine bases or mismatches at positions 13 and/or 15 of the guide strand. ARPE-19 cells were transfected with 10 nM duplex RNA and VEGF-A (ELISA) was assayed 48 hours post-transfection. Each bar represents the mean of triplicate samples. Error bars indicate standard deviation.