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Table 2 Summary of purification of rBmNPV-hPRR from hemolymph by Superdex 200 10/300 GL and Sephacryl S-1000 SF column chromatography

From: High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization

  Volume (ml) Titer (pfu/ml) Virus (pfu) Protein (mg/ml) Virus/Protein (pfu/mg) Recovery (%) Purification (fold)
Hemolymph 0.5 2.38E+08 1.19E+08 33.2 7.17E+06 100 1
Superdex 200 10/300 GL 2 6.05E+07 1.21E+08 0.21 2.88E+08 102 40
Concentrated virus 0.25 1.44E+08 3.60E+07 0.42 3.43E+08 30 48
Hemolymph 7 2.91E+08 2.04E+09 24.2 1.20E+07 100 1
Sephacryl S-1000 30 2.40E+07 7.21E+08 0.024 1.00E+09 35 83
Concentrated virus 0.5 1.60E+08 8.00E+07 0.073 2.19E+09 4 183