Table 2 Summary of purification of rBmNPV-hPRR from hemolymph by Superdex 200 10/300 GL and Sephacryl S-1000 SF column chromatography
Volume (ml) | Titer (pfu/ml) | Virus (pfu) | Protein (mg/ml) | Virus/Protein (pfu/mg) | Recovery (%) | Purification (fold) | |
|---|---|---|---|---|---|---|---|
Hemolymph | 0.5 | 2.38E+08 | 1.19E+08 | 33.2 | 7.17E+06 | 100 | 1 |
Superdex 200 10/300 GL | 2 | 6.05E+07 | 1.21E+08 | 0.21 | 2.88E+08 | 102 | 40 |
Concentrated virus | 0.25 | 1.44E+08 | 3.60E+07 | 0.42 | 3.43E+08 | 30 | 48 |
Hemolymph | 7 | 2.91E+08 | 2.04E+09 | 24.2 | 1.20E+07 | 100 | 1 |
Sephacryl S-1000 | 30 | 2.40E+07 | 7.21E+08 | 0.024 | 1.00E+09 | 35 | 83 |
Concentrated virus | 0.5 | 1.60E+08 | 8.00E+07 | 0.073 | 2.19E+09 | 4 | 183 |