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Figure 3 | BMC Biotechnology

Figure 3

From: High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization

Figure 3

Purification of rBmNPV-hPRR from hemolymph by Superdex 200 10/300 GL column chromatography and Sephacryl S-1000 SF column chromatography. (A) and (C) Chromatogram of Superdex 200 10/300 GL and Sephacryl S-1000 SF column chromatography and GP64 detection by Western blot using anti-Bmgp64 antibody. Solid and dotted lines indicate absorbance at 280 and 254 nm, respectively. Numbers on GP64 bands indicate fraction numbers. (B) and (D) Baculovirus number as present in each fraction of Superdex 200 10/300 GL and Sephacryl S-1000 SF column chromatography. (E) SDS-PAGE analysis of proteins of each purified rBmNPV-hPRR fraction stained with CBB. Lane 1, hemolymph; lane 2, rBmNPV-hPRR purified by Superdex 200 10/300 GL column chromatography; lane 3, rBmNPV-hPRR purified by Sephacryl S-1000 column chromatography; lane 4, rBmNPV-hPRR purified from Bm5 cell culture supernatant.

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