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Table 1 Purification of a recombinant FLAG-tagged ST6Gal1 from silkworm larval hemolymph

From: Synthesis of sialoglycopolypeptide for potentially blocking influenza virus infection using a rat α2,6-sialyltransferase expressed in BmNPV bacmid-injected silkworm larvae

 

Total activity (Ua)

Total protein (mg)

Specific activity (U/mg protein)

Recovery yield (%)

Purification (fold)

Crude enzyme

(larval hemolymph)

9.0

550.0

0.016

100

1

(NH4)2SO4 (25–70%)

7.2

105.0

0.07

80

4

Anti-FLAG M2 affinity

5.8

2.2

2.60

64

159

  1. a One unit of activity is defined as 1 μmol/min of transfer product 5 formed from compound 3 as acceptor substrate under the condition of 37°C and pH 7.4. Product 5 and compound 3 were measured by HPLC-based assay described in Materials and Methods.
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BMC Biotechnology

ISSN: 1472-6750

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