Grouping of clones by high resolution melt curve analysis (HRMA). A) Normalized and temperature shifted melt curves for the 25 samples that showed a positive result with ELISA. Samples with differences in their DNA sequences can be easily distinguished because of their different shaped melt curves. The different groups as assigned by HRMA are represented by different colours. B) Shows the same samples as in A. The differences in melt curve shapes are further analysed by using the sample with the highest melting temperature from the normalized and temperature shifted melt curves as a baseline in order to cluster samples automatically into groups that have similar melt curves. The different groups as assigned by HRMA are represented by different colours.