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Table 1 Physical properties of SiO2, DODAB BF dispersion, BSA, 18 kDa-hsp leprae and their mixtures in 1 mM NaCl (pH 6.3) or in 5 mM TrisHCl (pH 7.4)

From: Silica-based cationic bilayers as immunoadjuvants

Dispersion SiO2
(mg/ml)
DODAB
(mM)
Protein
(mg/ml)
Mean Diameter
(nm)
Zeta Potencial
(mV)
Polydispersity
(Index)
SiO2(i) 0.1 - - 328 ± 7 -40 ± 2 0.240 ± 0.01
DODAB(i) - 1.0 - 67 ± 1 41 ± 3 0.120 ± 0.04
BSA(i) - - 0.5 25 ± 4 -28 ± 1 0.450 ± 0.09
DODAB/BSA(i) - 0.1 0.500 72 ± 1 3 ± 1 0.245 ± 0.01
SiO2/DODAB(i) 0.1 0.05 - 304 ± 2 32 ± 1 0.260 ± 0.02
SiO2/DODAB/BSA(i) 0.1 0.05 0.025 324 ± 8 24 ± 1 0.301 ± 0.01
SiO2(ii) 0.1 - - 294 ± 2 -47 ± 2 0.180 ± 0.02
DODAB(ii) - 1.0 1.0 74 ± 1 33 ± 5 0.270 ± 0.04
BSA(ii) - - 0.5 24 ± 1 -58 ± 18 0.397 ± 0.01
SiO2/DODAB(ii) 0.1 0.05 - 373 ± 7 33 ± 2 0.260 ± 0.02
SiO2/DODAB/BSA(ii) 0.1 0.05 0.025 851 ± 36 -1.3 ± 4 0.396 ± 0.03
18 kDa-hsp M. leprae(i) - - 0.5 732 ± 188 -29 ± 2 0.35 ± 0.05
DODAB/18 kDa-hsp(i) - 0.1 0.05 208 ± 8 39 ± 5 0.315 ± 0.01
18 kDa-hsp M. leprae(ii) - - 0.05 360 ± 16 -43 ± 18 0.180 ± 0.09
SiO2/DODAB/18 kDa-hsp(ii) 0.1 0.05 0.05 2132 ± 370 -2 ± 2 0.492 ± 0.04
Al(OH3)(i) - - - 458 ± 3 28 ± 3 0.191 ± 0.04
Al(OH3)(ii) - - - 702 ± 17 14 ± 2 0.317 ± 0.01
Al(OH3)/18 kDa-hsp(i),(iii) - - 0.05 4888 ± 665 - 0.272 ± 0.07
Al(OH3)/18 kDa-hsp(ii),(iii) - - 0.05 10607 ± 2998 - 0.480 ± 0.03
  1. (i) 1 mM NaCl (pH 6.3). (ii) 5 mM Tris-HCl (pH 7.4). (iii) Al(OH3) at 0.1 mg/ml.