Table 1 Physical properties of SiO2, DODAB BF dispersion, BSA, 18 kDa-hsp leprae and their mixtures in 1 mM NaCl (pH 6.3) or in 5 mM TrisHCl (pH 7.4)
Dispersion | SiO2 (mg/ml) | DODAB (mM) | Protein (mg/ml) | Mean Diameter (nm) | Zeta Potencial (mV) | Polydispersity (Index) |
|---|---|---|---|---|---|---|
SiO2(i) | 0.1 | - | - | 328 ± 7 | -40 ± 2 | 0.240 ± 0.01 |
DODAB(i) | - | 1.0 | - | 67 ± 1 | 41 ± 3 | 0.120 ± 0.04 |
BSA(i) | - | - | 0.5 | 25 ± 4 | -28 ± 1 | 0.450 ± 0.09 |
DODAB/BSA(i) | - | 0.1 | 0.500 | 72 ± 1 | 3 ± 1 | 0.245 ± 0.01 |
SiO2/DODAB(i) | 0.1 | 0.05 | - | 304 ± 2 | 32 ± 1 | 0.260 ± 0.02 |
SiO2/DODAB/BSA(i) | 0.1 | 0.05 | 0.025 | 324 ± 8 | 24 ± 1 | 0.301 ± 0.01 |
SiO2(ii) | 0.1 | - | - | 294 ± 2 | -47 ± 2 | 0.180 ± 0.02 |
DODAB(ii) | - | 1.0 | 1.0 | 74 ± 1 | 33 ± 5 | 0.270 ± 0.04 |
BSA(ii) | - | - | 0.5 | 24 ± 1 | -58 ± 18 | 0.397 ± 0.01 |
SiO2/DODAB(ii) | 0.1 | 0.05 | - | 373 ± 7 | 33 ± 2 | 0.260 ± 0.02 |
SiO2/DODAB/BSA(ii) | 0.1 | 0.05 | 0.025 | 851 ± 36 | -1.3 ± 4 | 0.396 ± 0.03 |
18 kDa-hsp M. leprae(i) | - | - | 0.5 | 732 ± 188 | -29 ± 2 | 0.35 ± 0.05 |
DODAB/18 kDa-hsp(i) | - | 0.1 | 0.05 | 208 ± 8 | 39 ± 5 | 0.315 ± 0.01 |
18 kDa-hsp M. leprae(ii) | - | - | 0.05 | 360 ± 16 | -43 ± 18 | 0.180 ± 0.09 |
SiO2/DODAB/18 kDa-hsp(ii) | 0.1 | 0.05 | 0.05 | 2132 ± 370 | -2 ± 2 | 0.492 ± 0.04 |
Al(OH3)(i) | - | - | - | 458 ± 3 | 28 ± 3 | 0.191 ± 0.04 |
Al(OH3)(ii) | - | - | - | 702 ± 17 | 14 ± 2 | 0.317 ± 0.01 |
Al(OH3)/18 kDa-hsp(i),(iii) | - | - | 0.05 | 4888 ± 665 | - | 0.272 ± 0.07 |
Al(OH3)/18 kDa-hsp(ii),(iii) | - | - | 0.05 | 10607 ± 2998 | - | 0.480 ± 0.03 |