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Table 8 Primers used in this study

From: Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans

Primer

Sequence

FLEGSp

5'-pTCGATGACGGCAGATTTAGAGTCTGCTNNNTTTGGCTATAGTGAGTCGTATTAATTTCG-3'

RLEGSp

5'-pCTTCGAAGGTTCGAATCCTTCNNNNNNNACCATTTTAGCATAACCCCTTGGGGCCTCT-3'

FEGSp-Ngfp-lacZ

5'p-TCGATGACGGCAGATTTAGAGTCTGCTTTCTTTGGCTATAGTGAGTCGTATTAATTTCG-3'

REGSp-Ngfp-lacZ

5'p-CTTCGAAGGTTCGAATCCTTCTTTAAATACCATTTTAGCATAACCCCTTGGGGCCTCT-3'

FEGSp-Mtgfp

5'p-TCGATGACGGCAGATTTAGAGTCTGCTATCTTTGGCTATAGTGAGTCGTATTAATTTCG-3'

REGSp-Mtgfp

5'p-CTTCGAAGGTTCGAATCCTTCTATTAACACCATTTTAGCATAACCCCTTGGGGCCTCT-3'

FEGSp-Ngfp-lacZ-D

5'p-TCGATGACGGCAGATTTAGAGTCTGCTTTCTTTGGCTATAGTGAGTCGTATTAATTTCG-3'

REGSp-Ngfp-lacZ-D

5'p-CTTCGAAGGAAGGAATCCTTCTTTAAATACCATTTTAGCATAACCCCTTGGGGCCTCT-3'

FEGSp-Mtgfp-D

5'p-TCGATGACGGCAGATTTAGAGTCTGCTATCTTTGGCTATAGTGAGTCGTATTAATTTCG-3'

REGSp-Mtgfp-D

5'p-CTTCGAAGGAAGGAATCCTTCTATTAACACCATTTTAGCATAACCCCTTGGGGCCTCT-3'

FLEGSp-35-D

5'-pTCGATGACGGCAGATTTAGAGTCTGCTTCCTTTGGCTATAGTGAGTCGTATTAATTTCG-3'

RLEGSp-35-D

5'-pCTTCGAAGGAAGGAATCCTTCATGCGACACCATTTTAGCATAACCCCTTGGGGCCTCT-3'

FLEGSp-83-D

5'-pTCGATGACGGCAGATTTAGAGTCTGCTTTGTTTGGCTATAGTGAGTCGTATTAATTTCG-3'

RLEGSp-83-D

5'-pCTTCGAAGGAAGGAATCCTTCTATGTTCACCATTTTAGCATAACCCCTTGGGGCCTCT-3'

Fclone-IVTT

5'-AAATGGTNNNNNNNGAAG-3'

Rclone -IVTT

5'-AGATTGTGCACCGCCGCT-3'

FNgfp-lacZ-IVTT

5'-AAATGGTATTTAAAGAAGGA-3'

RNgfp-lacZ-IVTT

5'-AGATTGTGCACCGCCGCT-3'

FMtgfp-IVTT

5'-AAATGGTGTTAATAGAAGGA-3'

RMtgfp-IVTT

5'-AGATTGTGCACCGCCGCT-3'

F35-IVTT

5'-AAATGGTGTCGCATGAAG-3'

R35 -IVTT

5'-AGATTGTGCACCGCCGCT-3'

F83-IVTT

5'-AAATGGTGAACATAGAAG-3'

R83 -IVTT

5'-AGATTGTGCACCGCCGCT-3'

eft-2-QPCR-F

5'-GACGAGAAGGATTTGGAAGGAA-3'

eft-2-QPCR-R

5'-ACTGGGGATGGAAGATGGAA-3'

GFP-QPCR-F

5'-TGGAGTTGTCCCAATTCTTGTT-3'

GFP-QPCR-R

5'-GCATCACCTTCACCCTCTCC-3'

S-EGS-F

5'-TTAGAGCTTGACGGGGAAAG-3'

S-EGS-R

5'-CCTGCCACCATACCCACGCC-3'

  1. The "p" in the "sequence" column represents the modification by phosphorylation. Fclone-IVTT is the outline of the corresponding primer used in the specific experiment. Base-substitution mutations at three positions of the T-loop are indicated by bold text.