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Table 2 Microarray hybridization protocol used in an automated HS-400 hybridization station

From: Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications

   Temp. C Duration Repetitions
1 Prewash 85 Wash: 60 s; Soak: 30 s 1
2 Probe injection 34   1
3 Hybridization 34 4 h 00 min, High agitation 1
4 1. wash 23 Wash: 90 s; Soak: 30 s 3
5 2. wash 23 Wash: 90 s; Soak: 30 s 3
6 3. wash 23 Wash: 90 s; Soak: 30 s 3
7 Slide drying 23 90 s 1
  1. 1. Prewash – 6× saline sodium citrate (SSC); 0,5% Na-dodecyl sulphate (SDS)
  2. 2. Hybridization – 6× SSC; 0,5% SDS and 5× Denhardts solution
  3. 3. 1. wash – 2× SSC; 0,03% SDS
  4. 4. 2. wash – 1× SSC
  5. 5. 3. wash – 0,2× SSC