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Table 2 Microarray hybridization protocol used in an automated HS-400 hybridization station

From: Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications

  

Temp. C

Duration

Repetitions

1

Prewash

85

Wash: 60 s; Soak: 30 s

1

2

Probe injection

34

 

1

3

Hybridization

34

4 h 00 min, High agitation

1

4

1. wash

23

Wash: 90 s; Soak: 30 s

3

5

2. wash

23

Wash: 90 s; Soak: 30 s

3

6

3. wash

23

Wash: 90 s; Soak: 30 s

3

7

Slide drying

23

90 s

1

  1. 1. Prewash – 6× saline sodium citrate (SSC); 0,5% Na-dodecyl sulphate (SDS)
  2. 2. Hybridization – 6× SSC; 0,5% SDS and 5× Denhardts solution
  3. 3. 1. wash – 2× SSC; 0,03% SDS
  4. 4. 2. wash – 1× SSC
  5. 5. 3. wash – 0,2× SSC
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BMC Biotechnology

ISSN: 1472-6750

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