Table 1 Primer sequences used for dsRNA synthesis, Reat-time RT PCR and wpgrp1 cDNA amlification. The nucleotide in bold are from the T7 RNA polymerase promoter.

Primers used for dsRNA synthesis

5'pgrp1-T7dsRNA

5'-TAATACGACTCACTATAGGGCCAGTCCCTTACGTCGTCAT-3'

3'pgrp1-T7dsRNA

5'-TAATACGACTCACTATAGGGTCTGTTTCTCGGACTTGCCT-3'

5'gfp-T7dsRNA 5'

5'-TAATACGACTCACTATAGGGCAAGGAGGACGGCAACATCC-3'

3'gfp-T7dsRNA

5'TAATACGACTCACTATAGGGATTTTATGTTTCAGGTTCAG-3'

Primers used for Real-time RT PCR transcript amplification

wpgrp1-For

5'-ATAATTTCGCTGTTGGAGGG-3'

wpgrp1-Rev

5'-TCTCGGACTTGCCTATGACC-3'

gapdh-For

5'-AACTTTGCCGACAGCCTTGG-3'

gapdh-Rev

5'-GCGCCCATGTATGTAGTTGG-3'

Primers uses for PCR amplification of wpgrp1 cDNA

For-wpgrp1 cDNA

5'-ATGTCCAGTAAGCAATCACGG-3'

Rev-wpgrp1 cDNA

5'-TAACACAATTAGAGTGAAAGATAG-3'