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Table 1 Primer sequences used for dsRNA synthesis, Reat-time RT PCR and wpgrp1 cDNA amlification. The nucleotide in bold are from the T7 RNA polymerase promoter.

From: RNAi in the cereal weevil Sitophilusspp: Systemic gene knockdown in the bacteriome tissue

Primers used for dsRNA synthesis
5'pgrp1-T7dsRNA 5'-TAATACGACTCACTATAGGGCCAGTCCCTTACGTCGTCAT-3'
3'pgrp1-T7dsRNA 5'-TAATACGACTCACTATAGGGTCTGTTTCTCGGACTTGCCT-3'
5'gfp-T7dsRNA 5' 5'-TAATACGACTCACTATAGGGCAAGGAGGACGGCAACATCC-3'
3'gfp-T7dsRNA 5'TAATACGACTCACTATAGGGATTTTATGTTTCAGGTTCAG-3'
Primers used for Real-time RT PCR transcript amplification
wpgrp1-For 5'-ATAATTTCGCTGTTGGAGGG-3'
wpgrp1-Rev 5'-TCTCGGACTTGCCTATGACC-3'
gapdh-For 5'-AACTTTGCCGACAGCCTTGG-3'
gapdh-Rev 5'-GCGCCCATGTATGTAGTTGG-3'
Primers uses for PCR amplification of wpgrp1 cDNA
For-wpgrp1 cDNA 5'-ATGTCCAGTAAGCAATCACGG-3'
Rev-wpgrp1 cDNA 5'-TAACACAATTAGAGTGAAAGATAG-3'